==== SMRTAnalysis ==== === installation === - add SMRTAnalysis_HOME to your .bashrc:echo "export SMRTAnalysis_HOME=/home/lv60000/markus/downloads/SMRT-Analysis" >> ~/.bashrc source ~/.bashrc - Run the installer, use default optionx except for the points below: cd $SMRTAnalysis_HOME ./smrtanalysis_2.3.0.140936.run #DNS name: localhost #TOMCAT port: 8090 (8080 in use by cmd) #TMP directory [/tmp/smrtanalysis] ??? #job management system: NONE #cores used: 16 - Reset MYSQL password: cd $SMRTAnalysis_HOME ./smrtanalysis/admin/bin/smrtportald-initd start export LD_LIBRARY_PATH=$LD_LIBRARY_PATH:${SMRTAnalysis_HOME}/smrtanalysis/install/smrtanalysis_2.3.0.140936/redist/mysql-5.1.73/lib/mysql/ ${SMRTAnalysis_HOME}/smrtanalysis/install/smrtanalysis_2.3.0.140936/redist/mysql/bin/mysqladmin -u root -S /tmp/mysql_53306.sock password 'asdf' - edit '[client]' section of ./smrtanalysis/userdata/database/mysql/config/etc/mysql/my.cnf: [client] user = root password = yourpassword === running === - allocate a node: salloc -p mem_0128 --qos=normal_0128 - start server on allocated: srun $SMRTAnalysis_HOME/smrtanalysis/admin/bin/smrtportald-initd start - create SSH tunnel * on the login node: echo "ssh -L8090:$SLURM_JOB_NODELIST:8090 `whoami`@vsc3.vsc.ac.at" * on your local machine: paste the command from the previous step and create the SSH tunnel - navigate to http://localhost:8090/ in your local browser - when finished stop the server on the compute node: srun $SMRTAnalysis_HOME/smrtanalysis/admin/bin/smrtportald-initd stop - follow the instructions on [[http://www.pacb.com/wp-content/uploads/2015/09/SMRT-Analysis-Software-Installation-v2.3.0.pdf]]